Characterization of two different acyl carrier proteins in complex I from Yarrowia lipolytica
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چکیده
منابع مشابه
Characterization of an Extracellular lipase from Yarrowia lipolytica
Lipases from different sources have distinct properties, such as specificity, stability and optimal operational conditions. Moreover, different conditions of lipase production may alter these enzyme properties. Therefore, the aims of this study were related to the evaluation of lipase activity of Yarrowia lipolytica IMUFRJ 50682 (isolated from Baía de Guanabara, Brazil) produced under submerged...
متن کاملAcyl-CoA oxidase is imported as a heteropentameric, cofactor-containing complex into peroxisomes of Yarrowia lipolytica
Five isoforms of acyl-CoA oxidase (Aox), designated Aox1p to Aox5p, constitute a 443-kD heteropentameric complex containing one polypeptide chain of each isoform within the peroxisomal matrix of the yeast Yarrowia lipolytica. Assembly of the Aox complex occurs in the cytosol and precedes its import into peroxisomes. Peroxisomal targeting of the Aox complex is abolished in a mutant lacking the p...
متن کامل3D Reconstruction of a Subcomplex of NADH-ubiquinone-oxidoreductase (Complex I) from Yarrowia lipolytica.
Complex I is the first and largest enzyme in the respiratory chain located in the inner mitochondrial membrane and in the membrane of many bacteria [1]. In the process of oxidizing NADH, Complex I translocates 4 protons across the membrane. This is followed by additional proton translocation by complexes III and IV. The resulting membrane potential powers ATP production by the F-ATPase. The min...
متن کامل3D Reconstruction of the NADH:Ubiqinone Oxidoreductase (Complex I) from Yarrowia lipolytica Lacking Subunit NUMM
Complex I is the first enzyme of the respiratory chain in both the bacterial and the mitochondrial inner membranes. The mitochondrial enzyme from the aerobic yeast Yarrowia lipolytica has a molecular weight close to 1 MDa and contains at least 42 subunits. Complex I in all species exhibits an L-shape with two arms of similar length, one embedded in the membrane (membrane arm) and the other reac...
متن کاملSuperoxide radical formation by pure complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica.
Generation of reactive oxygen species (ROS) is increasingly recognized as an important cellular process involved in numerous physiological and pathophysiological processes. Complex I (NADH:ubiquinone oxidoreductase) is considered as one of the major sources of ROS within mitochondria. Yet, the exact site and mechanism of superoxide production by this large membrane-bound multiprotein complex ha...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 2010
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2009.09.007